Protein A Agarose CL-4B
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| Product Description | Protein A binds to the immunoglobulin G and M of many mammalian species. The product is protein A chemically linked to beaded agarose through a stable covalent linkage. The immobilized protein A on the beaded agarose could be utilized for the immunoprecipitation and purification of both polyclonal and monoclonal antibodies from mammalian species. |  | |
Formulation | 10 mg;1 mL Agarose Beads suspended in 2 ml slurry in glycerol | ||
| Ligand Immobilized | 10 mg protein A/mL matrix.The matrix linking chemistry is stable when eluted with weak acid (pH 2-3) and could be re-usable with no apparent loss of IgG binding capacity. | ||
| Binding Capacity | 30-40 mg rabbit IgG/mL beads as tested using rabbit serum | ||
| Applications | IP, IAC | ||
| Scientific Description | Protein A agarose consists of the native protein A immobilized on 4% cross linked agarose beads. It is made for the binding of immunoglobulins. Protein A is very heat and acid stable. | ||
| Storage & Stability | Product is stable for 30 days at ambient temperature For extended storage store product at 4 to–20ºC. | ||
| Product Specific References | 1. Trends Biochem. Sci. 1982.7: 74-76.doi:10.1016/0968-0004(82)90082-2 2. Rowan University School of Osteopathic Medicine. 2019. YfmK is a Novel Ne-lysine Acetyltransferase that Directly Acetylates the Histone-like Protein HBsu in Bacilius subtilis | ||
