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当前位置: 首页 > 产品中心 > Cryopreservation_reagent > ImmunoChem/Phospho-Crosstide Antibody/100 µg/ICP0190
商品详细ImmunoChem/Phospho-Crosstide Antibody/100 µg/ICP0190
ImmunoChem/Phospho-Crosstide Antibody/100 µg/ICP0190
ImmunoChem/Phospho-Crosstide Antibody/100 µg/ICP0190
商品编号: ICP0190
品牌: immunechem
市场价: ¥7500.00
美元价: 3750.00
产地: 美国(厂家直采)
公司:
产品分类: 冻存试剂
公司分类: Cryopreservation_reagent
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Phospho-Crosstide Antibody

Catalog # Pack Size Price(USD)
ICP0190 100 µg $375.00

Quantity:

Product Description
Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. Affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays.
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Phospho-crosstide (PRAApTF)-KLH
Purification
The antibody was affinity purified with PRAApTF on agarose followed by affinity absorption with the non-phosphorylated crosstide.
Conjugation
Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin).
Specificity
The antibody only recognizes the phosphorylated crosstide, the product of SGK and PKB in a non-radioactive kinase assay. There are no cross reactions with the non-phosphorylated crosstide.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive AKT and SGK kinase assay
Scientific Description
Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

1.Nature.1995.378: 785-789.doi:10.1038/378785a0

2.Biochem. J.1996.315: 709-713.

3.EMBO J.1996.15(23): 6541-6551.

品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年,公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础,以蛋白质分离提纯基础为核心,以免疫技术为应用,致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白,其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。