免疫化学/磷酸Crebtide抗体，HRP/100µg/ICP0182
商品编号：
ICP0182
品牌：
immunechem
市场价：
¥7500.00
美元价：
3750.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Phospho-Crebtide Antibody, HRP
|
Product Description | The affinity purified rabbit polyclonal anti-phospho-Crebtide (pCrebtide) antibodies are developed using a technique unique to ImmuneChem. The pCrebtide antibodies are affinity purified using phospho-Crebtide on agarose followed by affinity adsorption with the non-phospho-Crebtide. The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labeling of the primary anti-pCrebtide will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates and also facilitating the assay. The antibodies could be utilized for the detection and quantization of phosphorylated Crebtide by target protein kinases. |
Species | Rabbit |
Formulation | 250 µg/mL in stabilizing buffer with 50% glycerol |
Immunogen | Phospho-crebtide (KRREILSRRPpSYR)-KLH |
Purification | Affinity purified with KRREILSRRPpSYR on agarose followed by affinity absorption with non-phosphorylated crebtide |
Conjugation | Reductive amination; HRP/Ab molar ration - 2:1 |
Specificity | The antibody only recognizes the phosphorylated crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross reactions with non-phosphorylated crebtide. |
Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
Scientific Description | Creb (cAMP response element binding) proteins are transcription factors that regulate certain activities of gene transcription. Crebtide is a peptide (KRREILSRRPSYR) derived from the Creb protein. PKC kinases phosphorylate crebtide to form phospho-crebtide. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored. |
Product Specific References | 1. Cell. 1989.59(4): 675– 680.doi:10.1016/0092-8674(89)90013-5 |
品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年，公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础，以蛋白质分离提纯基础为核心，以免疫技术为应用，致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白，其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。
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