免疫化学/甲基化赖氨酸抗体，HRP/100μg/ICP0502
商品编号：
ICP0502
品牌：
immunechem
市场价：
¥7500.00
美元价：
3750.00
产品分类：
其它天然蛋白
公司分类：
Other_natural_proteins
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Methylated Lysine Antibody, HRP
|
Product Description | The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labelling of primary anti-MeK will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. The antibodies could be utilized for detection, quantization and isolation of proteins with MeK residues. |  Western blot analysis of the methylated protein pattern in whole HeLa cells with ICP0502. A. Signal of methylated proteins B. Signal of the methylated proteins was competitively suppressed by 50 µg/mL of methylated BSA C. Signal of the methylated proteins was competitively suppressed by 10 µg/mL of methylated BSA | |
Species | Rabbit | ||
Formulation | 500 µg/mL in HRP stabilizing buffer with 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | Methyl-lysine and di-methyl lysine on agarose | ||
Conjugation | Reductive amination; HRP/Ab molar ratio - 10:1 | ||
Specificity | Anti-MeK peroxidase conjugates recognize proteins methylated on their lysine residues (mono and di-methyllysine). They do not cross react with acetylated proteins. | ||
Applications | ELISA; WB | ||
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. | ||
Product Specific References | 1.J. Biol. Chem. 2003.278:18346-18352.doi.10.1074/jbc.M300890200 2.J. Biol. Chem. 2002.277:34655-34657.doi:10.1074/jbc.C200433200 3.J. Biol. Chem.2002.277:11621-11624.doi:10.1074/jbc.C200045200 |
品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年，公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础，以蛋白质分离提纯基础为核心，以免疫技术为应用，致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白，其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。
联络我们
