免疫化学/甲基化（ε-N）赖氨酸抗体/100µg/ICP0501
商品编号：
ICP0501
品牌：
immunechem
市场价：
¥7000.00
美元价：
3500.00
产品分类：
蛋白纯化试剂盒
公司分类：
Protein_purification_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Methylated (ε-N) Lysine Antibody
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Product Description | The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues. |  Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine anti-MeK (ICP0501). Histones were isolated with cold 0.1M HCl extraction followed by: A: Blot with 0.5 µg/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 µg/mL B: Blot with 0.5 µg/mL of anti-MeK (ICP0501) in TBSt | |
Species | Rabbit | ||
Formulation | 250 µg/mL in Tris-phosphate buffer, 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose. | ||
Specificity | The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methyllysine). There are no cross reactions with acetylated proteins. The cross reactivity with tri-methyl lysine has not been tested. | ||
Applications | ELISA; WB; IHC | ||
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] | ||
Storage & Stability | Store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 1. J. Biol. Chem. 2003. 278:18346-18352.doi.10.1074/jbc.M300890200 2.J. Biol. Chem. 2002. 277:34655-34657. doi:10.1074/jbc.C200433200 3.J. Biol. Chem.2002. 277:11621-11624. doi:10.1074/jbc.C200045200 4.Biochem & Biophys Res. Com.2014.451(2): 229-234.doi:10.1016/j.bbrc.2014.07.11 5.Mol. BioSyst.2013.9:2231-2247.doi:10.1039/C3MB00009E 6.Biochemical and Biophysical Research Communications Volume 451, Issue 2, 22 August 2014, Pages 229–234. doi: 10.1016/j.bbrc.2014.07.110. 7.Proteomics.2015.15(13): 2166–2176. doi:10.1002/pmic.201400521 8. BioRxiv2018. doi: 10.1101/240952 9.The FEBS Journal 2019.doi: 10.1111/febs.14867 10. EMBO Reports. 2019. 20(5): e43260. doi: 10.15252/embr.201643260
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品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年，公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础，以蛋白质分离提纯基础为核心，以免疫技术为应用，致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白，其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。
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