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当前位置: 首页 > 产品中心 > Protein_purification_kit > ImmunoChem/Methyl Lysine Antibody, Agarose/5 mg/ICP0588
商品详细ImmunoChem/Methyl Lysine Antibody, Agarose/5 mg/ICP0588
ImmunoChem/Methyl Lysine Antibody, Agarose/5 mg/ICP0588
ImmunoChem/Methyl Lysine Antibody, Agarose/5 mg/ICP0588
商品编号: ICP0588
品牌: immunechem
市场价: ¥17900.00
美元价: 8950.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白纯化试剂盒
公司分类: Protein_purification_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Methyl Lysine Antibody, Agarose

Catalog # Pack Size Price(USD)
ICP0588 5 mg $895.00

Quantity:

Product Description
The methylated lysine antibodies are developed with methylated KLH and affinity purified with methylated lysine on agarose. The purified antibodies are covalently immobilized to beaded agarose. The product could be utilized as an affinity matrix for rapid isolation, purification, and enrichment of the proteins or peptide with methylted lysine residues, particularly trimethyl- and/or dimethyl-lysine residues.
The ELISA titre (1:1620) of the methylated proteins eluted from 50 ul of the methyllysine-specific antibody affinity matrix (ICP0588) as detected with anti-methyllysine HRP (ICP0502). Approximately 50 mg of the swine spleen cell lysate were applied to the enrichment. ELISA signal can be inhibited methylated BSA (MeBSA).
Formulation
0.5 ml beaded agarose suspended in 1 ml slurry with glycerol
Antibody Immobilized
10 mg/ml of the methylated lysine antibodies are covalently linked to the agarose beads
Specificity
This antibody matrix selectively captures the peptides and proteins with methylated lysine residues (N-epsilon).
Binding Capacity
Approximately 0.5 mg of methylated histone/ml
Applications
Rapid isolation, purification, and affinity enrichment of peptides or proteins with the methylated lysine residues from mixtures of cell lysates or protease-digested samples.
Scientific Description
Protein methylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of the methylated protein/peptide is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the methylated species for further MS/MS identification.
Storage & Stability
Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Do not aliquot and shake thoroughly before use. Expiration date is one year from date of shipping of properly stored.

Product Specific References

  1. 1.Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E
  2. 2.Biochem & Biophys Res. Com. 2014. 451 (2): 229-234. doi: 10.1016/j.bbrc.2014.07.11
  3. 3.Proteomics. 2015. 15 (13): 2166–2176. doi: 10.1002/pmic.201400521

品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年,公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础,以蛋白质分离提纯基础为核心,以免疫技术为应用,致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白,其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。