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商品详细免疫化学/甲基(单)精氨酸抗体/100μg/ICP0801
免疫化学/甲基(单)精氨酸抗体/100μg/ICP0801
免疫化学/甲基(单)精氨酸抗体/100μg/ICP0801
商品编号: ICP0801
品牌: immunechem
市场价: ¥6500.00
美元价: 3250.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白交联剂
公司分类: Crosslinkers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Methyl (mono) Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0801 100 μg $325.00

Quantity:

Product Description
This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues.

Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1µg/mL) by free mMeR (IC50=40-50 ng/mL)

Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)

Fluorescent Staining of MMRU cells

Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
NG-mono-methyl arginine-KLH conjugates
Purification
Purified by antigen-specific affinity chromatography
Specificity

The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues. It does not cross react with either asymmetric or symmetric dimethyl arginine.

Species Tested
Pan-specific regardless of species.
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from the date of shipping if properly stored.

Product Specific References

  1. 1.J. Proteome Res.2013. 12(9): 3884-3899,doi:10.1021/pr400556c
  2. 2.Proteomics.2013.13(7): 1059-1064.doi:10.1002/pmic.201200477
  3. 3.Mol. Microbiol.2007.65(3): 590-606,doi:10.1111/j.1365-2958.2007.05831.x
  4. 4.Proteomics. 2015.15(18): 3209-3218.doi10.1002/pmic.201500075
  5. 5.Proteomics. 2016.16(3): 465-476. doi: 10.1002/pmic.201400564
  6. 6.J. Neurosci. 2015.35(37): 12890-12902.doi: 10.1523/JNEUROSCI.0246-15.2015

品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年,公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础,以蛋白质分离提纯基础为核心,以免疫技术为应用,致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白,其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。