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商品详细免疫化学/二甲基(ε-N)赖氨酸抗体,HRP/100μg/ICP0702
免疫化学/二甲基(ε-N)赖氨酸抗体,HRP/100μg/ICP0702
免疫化学/二甲基(ε-N)赖氨酸抗体,HRP/100μg/ICP0702
商品编号: ICP0702
品牌: immunechem
市场价: ¥7900.00
美元价: 3950.00
产地: 美国(厂家直采)
公司:
产品分类: 透析
公司分类: dialysis
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Di-methyl (ε-N) Lysine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0702 100 μg $395.00

Quantity:

Product Description
The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibodies are conjugated to horse radish peroxidase (HRP). The antibody-HRP conjugates could be utilized for detection, quantization and localization of proteins with Di-methylated lysine residues.
Western blot analysis of the di-methylated proteins from swine spleen cell lysate with (B) and without (A) the presence of methylated BSA, using anti-di-methyl lysine antibody HRP conjugates (anti-MeK HRP, ICP0702)
Species
Rabbit
Formulation
250 µg/mL in Tris-phosphate buffer with 1% BSA and 50% glycerol
Immunogen
Di-methylated KLH conjugates
Purification
The antibody is immunoaffinity purified with epsilon N-Di-methyl lysine on agarose.
Conjugation
Reductive amination with antibody/HRP molar ratio = 1:1
Specificity
The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested.
Applications
ELISA, WB
Scientific Description
Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example, calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.[1]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.

Product Specific References

  1. 1.Mol. BioSyst.2013.9: 2231-2247.doi: 10.1039/C3MB00009E
品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年,公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础,以蛋白质分离提纯基础为核心,以免疫技术为应用,致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白,其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。