ImmunoChem/Phosphotyrosine Antibody/100 µg/ICP9805
商品编号：
ICP9805
品牌：
immunechem
市场价：
¥6500.00
美元价：
3250.00
产品分类：
植物抗原
公司分类：
Plant_antigen
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Phosphotyrosine Antibody
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Product Description | The antibody is raised using the immunogenic conjugate phospho-tyrosine linked to KLH. The pan-specific antibodies for phospho-tyrosine were specifically and selectively isolated from the immune serum using a unique technique which makes sure the antibody can be competitively displaced by free phospho-tyrosine. This pan-specific anti-phospho-tyrosine (anti-pY) antibody could recognize the phospho-tyrosine residues on both proteins and peptides. It has proved to be useful for affinity isolation, localization and detection of tyrosine phosphorylated proteins. This antibody could be utilized for immunoprecipitation and affinity enrichment of tyrosine-phosphorylated proteins in cell and tissue lysates. It could be useful for immunoblotting tyrosine-phosphorylated proteins and ELISA quantization of protein tyrosine phosphorylation. It could also be used for non-radioactive tyrosine kinase assay. |  The affinity isolation and enrichment of the tyrosine-phosphorylated species in mouse spleen lysates with anti-phospho-tyrosine antibody (ICP9805), followed by immuno-blotting detection with this antibody conjugated to HRP (ICP9902).  Immunohistochemistry analysis of human breast cancer with ICP9805 | |
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3 | ||
Immunogen | Phospho-tyrosine KLH conjugates | ||
Purification | Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine | ||
Specificity | The antibody recognizes proteins and peptides phosphorylated on tyrosine residues. There are no cross reactions with phospho-serine or threonine. Tested on human breast cancer tissue. | ||
Applications | ELISA (1:1000); WB (1:250); IP (10 µg/500 µg protein sample); IHC (1:50); Non-radioactive Tyrosine Kinase Assay | ||
Scientific Description | Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References |
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品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年，公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础，以蛋白质分离提纯基础为核心，以免疫技术为应用，致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白，其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。
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