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商品详细免疫化学/磷酸丝氨酸抗体/100µg/ICP9806
免疫化学/磷酸丝氨酸抗体/100µg/ICP9806
免疫化学/磷酸丝氨酸抗体/100µg/ICP9806
商品编号: ICP9806
品牌: immunechem
市场价: ¥6500.00
美元价: 3250.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白酶类
公司分类: protease
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Phosphoserine Antibody

Catalog # Pack Size Price(USD)
ICP9806 100 µg $325.00

Quantity:

Product Description
Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.
Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):
Lane1-2: Kinase untreated
Lane 3-6: kinase treated with phosphatase PP1G
Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.
A: Control
B-D: Treatment with increased UV dose
E: Phosphatase inhibitor treatment
High affinity anti-phospho-serineantibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin andα-casein) but not non-phospho proteins (ovalbumin, BSA,β-lactoglobulin, trypsinogen)
Species
Rabbit
Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Phospho-serine KLH and phosvitin mixture

Purification

Affinity purified with phospho-tyrosine on agarose

Specificity

The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.

Tested

Human breast cancer tissue and mouse cell

Applications

ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)

Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

  1. 1. Curr. Opin. Cell. Bio. 2001. 13(2): 131-138.doi:10.1016/S0955-0674(00)00189-7
  2. 2.Nat. Commun. 2016. 7: 11151. doi:10.1038/ncomms11151
  3. 3.Nature Communications. 2016. 7:11151: 1-15. doi:I: 10.1038/ncomms11151
  4. 4.Front Immunol. 2017; 8: 352. doi: 10.3389/fimmu.2017.00352.
  5. 5. Front Immunol. 2017; 8: 189. doi: 10.3389/fimmu.2017.00189.
  6. 6. JAHA. 2018; 7(1) doi: 10.1161/JAHA.117.007185
  7. 7. AJHG. 2018. 102(4): 649-657. doi: 10.1016/j.ajhg.2018.02.015
  8. 8. JAHA. 2018. 7(1). doi: 10.1161/JAHA.117.007185
  9. 9. Cellular Physiology and Biochemisty. 2018. 46 (1). doi: 10.1159/000488418
  10. 10. Cell Reports. 2019. 28(3): 235-245. doi: 10.1016/j.celrep.2019.06.045
  11. 11. American Journal of Human Genetics. 2018. 102(4): 649-657. doi: 10.1016/j.ajhg.2018.02.015
  12. 12. Journal of Molecular and Cellular Cardiology. 2020. 139: 47-61. doi: 10.1016/j.yjmcc.2019.12.013

品牌介绍
加拿大免疫化学药剂有限公司(ImmuneChem Pharmaceuticals Inc. 简称ICP) 是由加拿大政府资助而创立的生物高科技开发公司。始创立于1997年,公司的研究与开发部门位于加拿大不列颠斯哥伦比亚省大温哥华地区。ICP是一个以蛋白质化学技术和免疫技术为基础的免疫化学药物高科技公司。以固相表面化学技术为基础,以蛋白质分离提纯基础为核心,以免疫技术为应用,致力于开发科研和诊断用免疫球蛋白以及医疗用被动免疫球蛋白,其所创建的ImmuneChem品牌抗体试剂多年来畅销世界各地。